Used in immunological research to quantify levels of Toll-like receptor 4 (TLR4) in biological samples such as cell lysates, tissue extracts, or serum. This kit enables researchers to study TLR4 expression in response to pathogens, inflammatory stimuli, or therapeutic agents. It is widely applied in studies related to innate immunity, sepsis, chronic inflammation, autoimmune diseases, and vaccine development. The assay helps evaluate activation of immune cells, particularly macrophages and dendritic cells, and supports drug discovery efforts targeting TLR4-mediated signaling pathways.