Used in molecular biology and genetic research to quantify TATA box binding protein (TBP) and its related factors (TAFs) in cell lysates or nuclear extracts. Helps investigate transcription initiation mechanisms, especially in studies related to gene expression regulation and RNA polymerase II activity. Commonly applied in studies of cellular differentiation, stress response, and cancer research where transcriptional dysregulation occurs. Enables high-throughput screening of transcription factor levels in response to drugs or genetic modifications. Suitable for research use only, not for diagnostic or therapeutic procedures.