Used in immunological research to quantify Bovine Tumor Necrosis Factor β (TNF-β) levels in biological samples such as serum, plasma, or cell culture supernatants. This kit enables researchers to study inflammatory responses in cattle, supporting investigations into infectious diseases like bovine tuberculosis or mastitis. It is also applied in veterinary diagnostics and vaccine development to monitor immune activation and cytokine production. The assay provides a sensitive and specific measurement, allowing for high-throughput analysis in experimental and clinical settings.