Used in biomedical research to quantify levels of tissue inhibitor of metalloproteinase-4 (TIMP-4) in rat samples, particularly in studies related to extracellular matrix remodeling. Commonly applied in cardiovascular research, as TIMP-4 plays a role in heart tissue regulation and repair. Also utilized in cancer studies to investigate tumor progression and metastasis, where imbalance between matrix metalloproteinases and their inhibitors affects tissue integrity. Enables researchers to assess TIMP-4 concentration in serum, plasma, or tissue homogenates with high sensitivity and specificity, supporting mechanistic insights in inflammatory diseases, fibrosis, and neurodegenerative conditions.