Used in biomedical research to quantify TIMP-1 protein levels in biological samples such as serum, plasma, and cell culture supernatants. Helps in studying tissue remodeling, inflammation, and cancer progression due to TIMP-1's role in regulating matrix metalloproteinases. Commonly applied in studies of liver disease, neurological disorders, and cardiovascular conditions where extracellular matrix degradation is involved. Provides a reliable, sensitive, and high-throughput method for detecting TIMP-1, supporting both clinical research and drug development.