Used in laboratory research to measure levels of thyroxine-releasing hormone (TRH) in rat samples. Helps in studying thyroid function, neuroendocrine pathways, and hypothalamic-pituitary-thyroid axis regulation in experimental models. Commonly applied in endocrinology, neuroscience, and toxicology studies to assess hormonal responses under various physiological or pathological conditions. Provides a sensitive and specific method for quantifying TRH in serum, plasma, or tissue extracts from rats.