Used in immunological research to quantify Rat Toll-like Receptor 9 (TLR-9) levels in biological samples such as serum, plasma, cell lysates, and tissue homogenates. This ELISA kit enables researchers to study TLR-9 involvement in innate immune responses, particularly in pathogen recognition, inflammation, and autoimmune diseases. It is widely applied in preclinical studies involving rodent models to evaluate immune activation in response to bacterial or viral DNA, assess vaccine efficacy, and investigate therapeutic interventions targeting TLR signaling pathways. The assay provides a sensitive and specific method for monitoring changes in TLR-9 expression under experimental conditions, supporting advances in immunology, infectious disease research, and drug development.