Used in immunological research to quantify Rat Toll-like Receptor 4 (TLR4) levels in biological samples such as serum, plasma, tissue homogenates, and cell culture supernatants. This ELISA kit enables researchers to study the role of TLR4 in inflammatory responses, innate immunity, and disease mechanisms including sepsis, autoimmune disorders, and neuroinflammation. It is commonly applied in preclinical studies involving rat models to evaluate immune activation and the effectiveness of therapeutic agents targeting the TLR4 signaling pathway. The assay provides a sensitive and specific means to measure TLR4 protein concentration, supporting investigations in pharmacology, toxicology, and molecular biology.