Used in clinical and research laboratories to measure free cortisol levels in human urine samples. This kit enables the assessment of cortisol excretion over time, which is critical for diagnosing and monitoring adrenal disorders such as Cushing's syndrome and Addison's disease. The assay supports studies related to stress response, hypothalamic-pituitary-adrenal (HPA) axis function, and metabolic disorders. Its high sensitivity and specificity make it suitable for large-scale epidemiological studies and longitudinal research where accurate quantification of urinary cortisol is required. The ELISA format allows for batch processing of samples, making it efficient for both research and diagnostic applications.