Used in biomedical research to quantify VEGF165 levels in cell cultures, tissue lysates, and biological fluids such as serum and plasma. Enables researchers to study angiogenesis, vascular development, and disease mechanisms including cancer, diabetic retinopathy, and inflammatory disorders. Supports evaluation of therapeutic agents targeting the VEGF pathway by providing a reliable method to measure changes in VEGF expression. Commonly used in preclinical studies, drug development, and biomarker validation.