Used in biomedical research to quantify vinculin protein levels in cell lysates or tissue samples. Helps study cell adhesion, migration, and cytoskeletal organization. Commonly applied in cancer research, cardiovascular studies, and developmental biology to assess changes in focal adhesion dynamics. Enables detection of vinculin expression under experimental conditions such as drug treatment, gene knockdown, or mechanical stress. Provides a reliable method for evaluating integrin-mediated signaling pathways in vitro and in vivo.