Used in biomedical research to quantify vascular endothelial growth factor (VEGF) levels in rat tissue samples and biological fluids. Enables researchers to study angiogenesis, vascular permeability, and endothelial function in experimental models. Commonly applied in studies of inflammation, cancer, ischemic diseases, and diabetic complications. Supports evaluation of therapeutic interventions targeting the VEGF signaling pathway. Provides a sensitive and specific method for monitoring changes in VEGF expression under various physiological and pathological conditions.