Used in biomedical research to detect and quantify phosphorylated TRPV4 protein levels in rat tissue samples or cell lysates. This assay helps study the activation state of TRPV4 channels, which play a role in cellular responses to mechanical and osmotic stress. Commonly applied in research on inflammation, pain sensation, cardiovascular function, and kidney physiology. Enables researchers to evaluate signaling pathways involving TRPV4 phosphorylation in experimental models.