Thymol blue is widely used as a pH indicator in various chemical and biological applications. It is particularly useful in titrations to determine the acidity or alkalinity of a solution, exhibiting distinct color changes at specific pH levels: red to yellow in the range of pH 1.2-2.8 (acidic) and yellow to blue in the range of pH 8.0-9.6 (alkaline). This property makes it valuable in laboratory settings for monitoring pH changes during experiments and detecting titration endpoints. Additionally, thymol blue is employed in microbiology to assess the pH of culture media, ensuring optimal conditions for microbial growth. It is also used in the food and pharmaceutical industries to check the acid-base properties of products, and in educational demonstrations to visually illustrate pH transitions in chemistry classes.