Methyl Red sodium salt is widely used as a pH indicator in various laboratory applications. It is particularly valuable in microbiology for differentiating bacterial species based on their metabolic activities. In the Methyl Red test, it helps identify enteric bacteria by detecting acid production during glucose fermentation. The chemical changes color from red at pH 4.4 to yellow at pH 6.2, making it a reliable tool for pH monitoring in titration experiments and biochemical assays. Additionally, it is employed in environmental studies to assess water quality and soil pH levels. Its stability and distinct color transition make it a preferred choice in educational and research settings for demonstrating pH changes.