Bromophenol Blue is widely used as a pH indicator in various laboratory applications. It changes color from yellow at pH 3.0 to blue at pH 4.6, making it useful for determining the acidity or alkalinity of solutions. In biochemistry and molecular biology, it is commonly added to DNA loading dyes to monitor the progress of electrophoresis, as it migrates at a similar rate to DNA fragments. Additionally, it is employed in titration experiments to visually detect the endpoint of acid-base reactions. Its vivid color change and stability make it a reliable tool in analytical chemistry and biological research.