Used as a complexing agent in analytical chemistry for the determination of metal ions like copper, nickel, and zinc. It forms colored complexes with these metals, enabling their detection and quantification through spectrophotometric methods. Applied in environmental monitoring to detect trace metal contamination in water and soil samples. Its sensitivity to low concentrations of metals makes it valuable for assessing pollution levels. Employed in biochemical research for studying metal-protein interactions. It helps in understanding the role of metal ions in biological systems and enzyme activities. Utilized in industrial quality control processes to ensure the purity of metal-based products and raw materials. Its ability to selectively bind to specific metals aids in accurate analysis. Also serves as a pH indicator in certain chemical reactions, providing visual cues for reaction progress or endpoint determination.