Dithizone is widely used in analytical chemistry as a chelating agent for the detection and determination of metal ions, particularly lead, zinc, cadmium, and mercury. It forms colored complexes with these metals, allowing for their identification and quantification through spectrophotometric methods. In environmental testing, dithizone is employed to analyze water and soil samples for heavy metal contamination. It is also utilized in biological research to study metal ion distribution in tissues and cells. Additionally, dithizone finds application in the pharmaceutical industry for quality control of metal-containing drugs and in the food industry to monitor metal impurities in products. Its selective reactivity with specific metals makes it a valuable tool in various scientific and industrial fields.