Murexide is widely used as a complexometric indicator in analytical chemistry, particularly in the titration of calcium and other metal ions. It changes color in the presence of specific metal ions, making it valuable for determining endpoints in EDTA titrations. Additionally, it serves as a pH indicator, transitioning from purple to yellow as pH decreases. In biological research, murexide is employed to study calcium ion dynamics in cells due to its ability to bind calcium selectively. It is also used in dyeing processes for textiles and as a colorant in certain industrial applications.