Dithizone is widely used as a complexing agent in analytical chemistry for the detection and determination of heavy metals, particularly lead, mercury, and zinc. It forms colored complexes with these metals, allowing for their identification and quantification through spectrophotometric methods. In environmental testing, dithizone is employed to monitor water and soil contamination by heavy metals. It is also utilized in biological research to study metal ion distribution in tissues and cells. Additionally, dithizone finds application in the pharmaceutical industry for quality control, ensuring the absence of toxic metal impurities in drug formulations. Its selective binding properties make it a valuable tool in various scientific and industrial processes.