6-Chloroisatin is widely used as an intermediate in the synthesis of various biologically active compounds. It plays a key role in the development of pharmaceuticals, particularly in the production of indole-based derivatives that exhibit antimicrobial, antiviral, and anticancer properties. Its structure serves as a core scaffold in the design of kinase inhibitors and other enzyme inhibitors relevant to cancer therapy. Additionally, it is employed in the preparation of dyes and pigments due to its stable aromatic framework and reactivity. The compound is also utilized in agrochemical research for developing new pesticides and herbicides with improved efficacy and selectivity.