Methyl nonacosanoate is primarily used in the field of entomology for studying insect behavior and communication. It serves as a key component in pheromone research, particularly in identifying and synthesizing compounds that mimic natural insect pheromones. This chemical is often employed in pest management strategies, where it helps in attracting or repelling specific insect species, thereby aiding in the control of agricultural pests. Additionally, it has applications in ecological studies to understand the interactions between insects and their environment, contributing to the development of environmentally friendly pest control methods.