Makisterone A is widely utilized in the field of entomology and insect physiology research. It serves as a crucial ecdysteroid hormone, playing a significant role in studying molting and metamorphosis processes in insects. Researchers employ it to better understand the hormonal regulation of growth and development in various arthropod species. Additionally, it is used in bioassays to evaluate the effects of ecdysteroids on cell differentiation and proliferation. Its application extends to the development of eco-friendly pest control strategies, as it helps in disrupting the normal growth cycles of harmful insects. In agriculture, it aids in managing insect populations by targeting their hormonal systems, reducing the reliance on traditional chemical pesticides. Furthermore, Makisterone A is explored in biotechnology for its potential in enhancing the production of bioactive compounds in cell cultures.