ZD7288 hydrate is primarily used in neuroscience research as a selective blocker of the hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, which are responsible for the Ih current. This compound is instrumental in studying the role of HCN channels in regulating neuronal excitability, rhythmic activity, and synaptic integration in the brain. It is particularly useful in exploring the mechanisms underlying pacemaker activity in the heart and brain, as well as in investigating conditions like epilepsy, pain, and sleep disorders. Additionally, ZD7288 hydrate is employed in electrophysiological experiments to understand the contribution of Ih current to neuronal signaling and network behavior. Its application extends to studies on learning and memory, where HCN channels are implicated in modulating synaptic plasticity.