Fluo-8, AM is widely used in biological research as a calcium indicator for live-cell imaging. Its cell-permeable nature allows it to easily enter cells, where it gets hydrolyzed by intracellular esterases to release the fluorescent dye Fluo-8. This dye binds to calcium ions, resulting in a significant increase in fluorescence intensity, making it a valuable tool for monitoring intracellular calcium dynamics. Researchers use it to study calcium signaling pathways, neuronal activity, and cellular responses to various stimuli. Its high sensitivity and bright fluorescence make it particularly suitable for detecting even subtle changes in calcium concentrations in real-time, aiding in the understanding of cellular functions and disease mechanisms.