IEF Anode Buffer is primarily used in isoelectric focusing (IEF) techniques, a critical method in protein analysis. It helps create a stable pH gradient necessary for separating proteins based on their isoelectric points. This buffer is applied at the anode end during electrophoresis, ensuring accurate and efficient protein separation. It is widely utilized in proteomics research, clinical diagnostics, and biotechnology for analyzing protein mixtures, identifying biomarkers, and studying post-translational modifications. Its role in maintaining consistent pH conditions enhances the reproducibility and reliability of IEF experiments.