Isoindoline-1-carboxylic acid hydrochloride is primarily used in organic synthesis and pharmaceutical research. It serves as a key intermediate in the development of various bioactive compounds, including potential drug candidates. The compound is often utilized in the synthesis of heterocyclic molecules, which are crucial in medicinal chemistry for creating drugs with therapeutic properties. Additionally, it finds application in the preparation of peptidomimetics, which mimic peptides and are used in drug design to enhance stability and bioavailability. Its role in constructing complex molecular structures makes it valuable in the exploration of new pharmacological agents.