Allobarbital-d10 is primarily used in research and analytical settings as an internal standard in mass spectrometry. Its deuterated form allows for precise quantification and identification of allobarbital in biological samples, ensuring accurate results in pharmacokinetic studies. This compound aids in understanding drug metabolism, distribution, and excretion, particularly in forensic and clinical toxicology. Its stable isotopic labeling minimizes interference, making it a reliable tool for detecting and measuring allobarbital levels in complex matrices like blood, urine, or tissue samples.