This compound is primarily used in research and clinical studies to investigate vitamin D metabolism and its role in various physiological processes. It serves as a deuterium-labeled analog of 1alpha, 25-dihydroxyvitamin D2, enabling precise tracking and quantification in analytical methods such as mass spectrometry. Researchers utilize it to study calcium and phosphate homeostasis, bone health, and immune system regulation. It is also instrumental in developing and validating assays for measuring vitamin D levels in biological samples, aiding in the diagnosis and management of vitamin D deficiency-related disorders. Additionally, it supports the development of therapeutic strategies targeting vitamin D pathways in conditions like osteoporosis, autoimmune diseases, and certain cancers.