Banoxantrone D12 dihydrochloride is primarily used in research and development, particularly in the field of oncology. It serves as a deuterated analog of banoxantrone, which is a compound studied for its potential anticancer properties. The incorporation of deuterium atoms enhances the stability of the molecule, making it useful in pharmacokinetic and metabolic studies. Researchers utilize this compound to track and analyze the distribution, metabolism, and excretion of banoxantrone in biological systems. This helps in understanding the drug's behavior and optimizing its therapeutic efficacy. Additionally, it is employed in the development of analytical methods, such as mass spectrometry, to ensure accurate detection and quantification of the parent drug and its metabolites in various biological samples.