GNE-049 is primarily utilized in the field of medicinal chemistry and drug development, particularly as a potent and selective inhibitor. It is often employed in research settings to study its effects on specific biological pathways or protein targets, aiding in the understanding of disease mechanisms. Its application extends to preclinical studies, where it is used to evaluate therapeutic potential in various conditions, such as cancer or inflammatory diseases. Researchers also leverage GNE-049 to explore structure-activity relationships, optimizing its properties for enhanced efficacy and safety in potential drug candidates. Additionally, it serves as a valuable tool in biochemical assays to investigate target engagement and inhibition kinetics.