Used in biomedical research to quantify the expression levels of the Na/H exchanger isoform 3 (NHE3) in human tissue samples and cell lysates. This ELISA kit enables researchers to study NHE3 activity in gastrointestinal and renal physiology, particularly in sodium absorption, pH regulation, and fluid balance. It is widely applied in studies related to hypertension, chronic kidney disease, and diarrheal disorders where NHE3 dysfunction plays a role. The assay supports high-throughput screening of drug candidates targeting ion transport mechanisms and helps evaluate the impact of therapeutic interventions on NHE3 protein levels in clinical and preclinical research.