ATI-2341 is primarily used in research settings for its potential role in modulating cellular signaling pathways associated with metabolic regulation and insulin sensitivity. It has been investigated for its ability to activate AMP-activated protein kinase (AMPK), a key enzyme involved in energy homeostasis, making it a compound of interest in studies related to type 2 diabetes and obesity. Due to its cell-permeable properties and stability, ATI-2341 is employed in in vitro and in vivo models to explore glucose uptake mechanisms and mitochondrial function. Its application remains confined to preclinical research, where it serves as a tool compound for validating therapeutic targets in metabolic diseases.