CHK1-IN-2 is primarily utilized in cancer research due to its role as a checkpoint kinase 1 (CHK1) inhibitor. It is employed in studies aimed at understanding the mechanisms of DNA damage response and cell cycle regulation. By inhibiting CHK1, it helps researchers explore how cancer cells respond to DNA damage, particularly in the context of chemotherapy and radiation therapy. This compound is also used to investigate potential synergies with other anticancer agents, as CHK1 inhibition can sensitize tumor cells to DNA-damaging treatments. Its application extends to the development of novel therapeutic strategies targeting cancer cells with defective DNA repair pathways.