Mps1-IN-1 is primarily used in research settings to study the role of Mps1 kinase, a key protein involved in cell division and chromosome alignment. It is employed as a selective inhibitor to investigate the mechanisms of mitosis, particularly in understanding how Mps1 kinase regulates the spindle assembly checkpoint. This chemical is valuable in cancer research, as inhibiting Mps1 can lead to errors in chromosome segregation, potentially inducing cell death in tumor cells. Researchers use Mps1-IN-1 to explore its potential as a therapeutic agent for targeting cancers with high mitotic activity. Additionally, it aids in studying drug resistance mechanisms and developing combination therapies for enhanced anticancer effects.