Mps1-IN-2 is primarily utilized in biochemical research as a selective inhibitor of the Mps1 (monopolar spindle 1) kinase, which plays a critical role in cell cycle regulation, particularly during the spindle assembly checkpoint. This compound is extensively used in studies aimed at understanding the mechanisms of chromosome segregation and mitotic processes. By inhibiting Mps1, researchers can investigate the consequences of checkpoint disruption, which may lead to chromosomal missegregation and aneuploidy. Such studies are crucial for exploring the role of Mps1 in cancer biology, as its overexpression is often observed in various tumor types. Additionally, Mps1-IN-2 serves as a tool compound in drug discovery efforts targeting Mps1 for potential anticancer therapies, helping to evaluate the efficacy and specificity of novel inhibitors in preclinical models.