PF-03814735 is primarily utilized in the field of cancer research, where it serves as a potent inhibitor of Aurora kinases, particularly Aurora A and B. These kinases play a crucial role in cell division and are often overexpressed in various types of cancer, making them attractive targets for therapeutic intervention. By inhibiting Aurora kinases, PF-03814735 disrupts the mitotic process, leading to the arrest of cell cycle progression and ultimately inducing apoptosis in cancer cells. This mechanism of action has been explored in preclinical studies for its potential to treat solid tumors and hematological malignancies. Additionally, PF-03814735 has been used as a tool compound in biochemical and cellular assays to further elucidate the biological functions of Aurora kinases and their roles in tumorigenesis. Its application extends to the development of novel cancer therapies, where it aids in the identification and validation of new drug targets.