PI-273 is primarily utilized in the field of biomedical research, particularly in the study of cancer and cell biology. It functions as a selective inhibitor targeting specific enzymes or proteins involved in cellular signaling pathways. Researchers employ PI-273 to investigate the mechanisms of cell proliferation, apoptosis, and metastasis, providing insights into potential therapeutic strategies for cancer treatment. Its application extends to drug discovery, where it serves as a tool compound to validate targets and assess the efficacy of novel inhibitors. Additionally, PI-273 is used in biochemical assays to study enzyme kinetics and protein interactions, contributing to a deeper understanding of molecular pathways in disease progression.