TC-Mps1-12 is primarily used in biochemical and cancer research as a potent and selective inhibitor of the Mps1 (Monopolar spindle 1) kinase. Mps1 plays a critical role in the spindle assembly checkpoint, a mechanism that ensures proper chromosome segregation during cell division. By inhibiting Mps1, TC-Mps1-12 disrupts this checkpoint, leading to errors in chromosome alignment and cell division. This makes it a valuable tool for studying the mechanisms of mitosis and the role of Mps1 in cancer progression. Additionally, it is explored for its potential as a therapeutic agent in cancer treatment, particularly in targeting tumors that rely on Mps1 for survival and proliferation. Its ability to induce chromosomal instability and cell death in cancer cells highlights its significance in oncology research.