CCT129957 is primarily used in research settings to study its effects on cellular processes, particularly in cancer biology. It acts as a selective inhibitor of Aurora kinases (A and B), making it valuable for investigating signaling pathways involved in cell proliferation and survival. Researchers utilize it to explore its potential in targeting cancer cells, such as in colorectal and lung cancers, and understanding mechanisms of resistance to therapy. Its application extends to preclinical studies aimed at developing novel therapeutic strategies for various malignancies.