CCT241533 dihydrochloride is primarily utilized in research settings as a potent and selective inhibitor of the Aurora kinase family, particularly Aurora A and Aurora B. Its application is significant in the study of cancer biology, as Aurora kinases play a crucial role in cell division and are often overexpressed in various cancers. By inhibiting these kinases, it helps researchers understand the mechanisms of tumor growth and progression. Additionally, it is used in preclinical studies to evaluate its potential as a therapeutic agent for treating cancers, particularly those resistant to other treatments. The compound is also employed in cellular assays to investigate the effects of Aurora kinase inhibition on cell cycle regulation, apoptosis, and mitosis.