WEE1-IN-4 is primarily used in research settings to study its potential as a therapeutic agent in cancer treatment. It functions as an inhibitor of the WEE1 kinase, a protein involved in cell cycle regulation. By targeting WEE1, this compound disrupts the DNA damage repair mechanism in cancer cells, leading to cell death. Its application is particularly significant in cancers with high replication stress, such as certain types of ovarian, breast, and glioblastoma cancers. Researchers are exploring its efficacy in combination with other chemotherapy agents or radiation therapy to enhance treatment outcomes. Additionally, WEE1-IN-4 is being investigated for its role in overcoming resistance to conventional therapies, offering a promising avenue for developing more effective cancer treatments.