WHI-P131 is primarily used in biochemical and pharmacological research as a selective inhibitor of tyrosine kinases, particularly targeting the Janus kinase (JAK) family. It is employed to study signaling pathways involved in cell proliferation, immune responses, and inflammation. Due to its ability to modulate kinase activity, WHI-P131 serves as a tool compound in cancer research, especially in hematological malignancies where JAK-STAT signaling is dysregulated. It also supports the development of more specific kinase inhibitors by providing insights into structure-activity relationships. Its application remains confined to laboratory and preclinical studies, aiding in the understanding of signal transduction mechanisms and potential therapeutic interventions.