Used primarily as a selective inhibitor of Rho-associated protein kinase (ROCK), this compound plays a key role in cell biology research. It helps maintain cell survival and prevent apoptosis, especially in single-cell cultures of human pluripotent stem cells. Commonly applied to improve the efficiency of cell attachment and proliferation in vitro. Widely used in regenerative medicine and tissue engineering to support clonal growth. Also utilized in studies involving cytoskeletal dynamics, cell motility, and smooth muscle contraction. Its ability to block ROCK signaling makes it valuable in research on neurological disorders, cardiovascular diseases, and cancer progression.