Z-JIB-04 is primarily utilized in research settings, particularly in the study of epigenetic regulation and cellular signaling pathways. It is known to act as a selective inhibitor of JumonjiC-domain-containing histone demethylases (e.g., KDM4A, KDM4B, KDM4C, KDM5B), making it a valuable tool for investigating the role of these enzymes in histone demethylation and various biological processes. Researchers often employ Z-JIB-04 to explore its effects on cell proliferation, differentiation, apoptosis, and gene expression, providing insights into potential therapeutic targets for diseases such as cancer. Additionally, its application in molecular biology experiments helps in understanding the mechanisms of epigenetic control and signal transduction within cells.