MARK substrate is widely used in biochemical and molecular biology research, particularly in enzyme activity assays. It serves as a specific substrate for kinases, enabling the study of phosphorylation processes, which are crucial for understanding cellular signaling pathways. Researchers utilize it to measure kinase activity, screen for kinase inhibitors, and investigate the mechanisms of kinase-related diseases. Its application extends to drug discovery, where it helps in identifying potential therapeutic compounds targeting kinase activity. Additionally, it is employed in diagnostic assays to detect abnormal kinase activity associated with certain cancers and other pathological conditions.