Used in biochemical research as a substrate for studying enzyme activity, particularly tyrosine kinases and phosphatases, due to its nitro groups which allow for easy detection and quantification. It serves as a model compound for investigating post-translational modifications in proteins. Additionally, it is employed in the development of analytical methods for detecting tyrosine derivatives in biological samples. Its stability and solubility in aqueous solutions make it suitable for use in various enzymatic assays and protein interaction studies.