Hexokinase from yeast is widely used in biochemical research and diagnostic applications. It plays a crucial role in glycolysis, catalyzing the phosphorylation of glucose to glucose-6-phosphate, making it essential for studying metabolic pathways. In clinical diagnostics, it is employed in glucose assays to measure blood sugar levels accurately. Additionally, it is utilized in enzyme kinetics studies to understand reaction mechanisms and enzyme behavior. Its lyophilized form ensures stability and ease of storage, making it convenient for laboratory use.