Nucleoside 5′-Diphosphate Kinase, sourced from bovine liver, is widely utilized in biochemical research and molecular biology for its role in nucleotide metabolism. It catalyzes the transfer of phosphate groups between nucleoside diphosphates and triphosphates, making it essential for maintaining nucleotide balance in cellular processes. This enzyme is often employed in enzymatic assays to study nucleotide-dependent reactions, DNA synthesis, and energy transfer mechanisms. Additionally, it is used in the preparation of labeled nucleotides for various research applications, including radiolabeling and fluorescence studies. Its stability and efficiency make it a valuable tool in investigating cellular signaling pathways and metabolic regulation.