Myokinase from rabbit muscle is primarily used in biochemical research to study enzyme kinetics and metabolic pathways. It plays a crucial role in catalyzing the transfer of phosphate groups between adenosine nucleotides, which is essential for understanding cellular energy metabolism. This enzyme is also employed in diagnostic assays to measure ATP and ADP levels, aiding in the assessment of cellular energy status. Additionally, it is utilized in the production of nucleotide analogs for pharmaceutical research, contributing to the development of drugs targeting metabolic disorders. Its application extends to biotechnology, where it is used in enzymatic synthesis and the modification of nucleotides for various industrial purposes.